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The Bradford protein assay (also known as the Coomassie protein assay) was developed by Marion M. Bradford in 1976. It is a quick and accurate [2] spectroscopic analytical procedure used to measure the concentration of protein in a solution.
The Bradford protein assay is a dye-binding assay based on the differential color change of a dye in response to various concentrations of protein. The dye reagents are commonly purchased from Bio-Rad (Richmond, CA).
Bradford assays are dye-binding assays for fast and simple protein quantification. The assay is performed at room temperature and no special equipment is required.
A rapid and accurate method for the estimation of protein concentration is essential in various areas of biology and biochemistry. An assay originally described by Bradford (1) has become the preferred method for quantifying protein in many laboratories. This...
The Bradford assay is very fast and uses about the same amount of protein as the Lowry assay. It is fairly accurate and samples that are out of range can be retested within minutes.
Bradford Assay. Bio-Rad's Bradford assays provide a simple and accurate method for determining protein concentrations. The binding of the Bradford reagent to proteins results in a color change which is measured with a spectrophotometer or a microplate reader. Choose the kit that meets your needs.
The Bradford assay relies on the binding of the dye Coomassie Blue G250 to protein. Detailed studies indicate that the free dye can exist in four different ionic forms for which the pK a values are 1.15, 1.82 and 12.4 (2).
