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26 Ogo 2022 · The spread plate method is a microbiological laboratory technique for isolating and counting the viable microorganisms present in a liquid sample by spreading a certain volume of the sample over an appropriate solidified culture media.
11 Mei 2012 · The spread plate technique is a viable counting method employed to plate a liquid sample to isolate or count the bacteria in that sample. A perfect spread plate technique will result in visible and countable colonies of bacteria evenly distributed on the plate.
Advantage: The spread plate method is useful for isolation of aerobic microorganisms. Bacterial culture is not exposed to high temperature as compare to pour plate method. Disadvantage: The spread plate method allows the growth of other microbes along with desired microbes.
10 Ogo 2022 · The spread plate technique involves using a sterilized spreader with a smooth surface made of metal or glass to apply a small amount of bacteria suspended in a solution over a plate. The plate needs to be dry and at room temperature so that the agar can absorb the bacteria more readily.
24 Ogo 2022 · Spread plate enumeration is a type of plate count method or standard count method of enumeration of microorganisms that only counts viable cells. In addition to enumeration, it also isolates the organisms from a mixed culture.
11 Mei 2012 · Here we present the steps for several plating methods routinely used in the laboratory to isolate, propagate, or enumerate microorganisms such as bacteria and phage. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials.
One of the main advantages of the spread plate method is its simplicity and speed. It requires fewer steps compared to the pour plate method, making it a preferred choice in situations where time is a constraint.
