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  1. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer designing tool - National Center for Biotechnology...

    Primer-BLAST allows you to design primers for PCR based on a template sequence, using Primer3 and BLAST algorithms. You can customize the search parameters, such as primer length, melting temperature, exon/intron selection, and specificity checking.

    • Publication

      Primer-BLAST was developed at NCBI to help users make...

    • Retrieve Recent Results

      Retrieve recent primer-blast jobs. Enter a job id: Last...

    • Blast

      The Basic Local Alignment Search Tool (BLAST) finds regions...

    • Primer-BLAST Help

      Primer-BLAST Help - Primer designing tool - National Center...

  2. www.ncbi.nlm.nih.gov › guide › howtoDesign PCR primers and check them for specificity

    Primer BLAST is a tool that helps you design and evaluate PCR primers based on a target sequence or a template sequence. You can enter one or both primer sequences, select the organism and database, and get primer pairs and specificity information.

  3. blast.ncbi.nlm.nih.gov › BlastBasic Local Alignment Search Tool - BLAST

    Nov 28, 2023 · The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

  4. www.ncbi.nlm.nih.gov › tools › primer-blastPrimer-BLAST - National Center for Biotechnology Information

    The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

  5. www.ncbi.nlm.nih.gov › tools › primer-blastNational Center for Biotechnology Information

    Primer-BLAST helps users make primers that are specific to intended PCR target by using Primer3 and BLAST algorithms. It screens primers against user-selected database to avoid non-specific amplifications and provides citation and details.

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  7. www.nlm.nih.gov › ncbi › workshopsThe Primer-BLAST interface - National Library of Medicine

    Oct 16, 2022 · The Primer-BLAST input form has four main sections: Sequence input (PCR Template and Primer Parameters) Exon / Intron selection. Primer Pair Specificity Checking Parameters (BLAST database selection and options) Advanced Parameters.

  8. www.nlm.nih.gov › ncbi › workshopsPractice Primer-BLAST Examples - National Library of Medicine

    We'll use Primer-BLAST to design primers for: A human transcript from the myeloperoxidase (MPO) gene and use the exon/intron selection to make them specific for the transcript. A specific exon of the human MPO gene that contains a clinically signficant SNP. Starting from the nucleotide record.