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The left sample contains chloride ions which quench quinine's fluorescence, so the left sample does not fluoresce visibly (the violet light is just scattered laser light). In chemistry, quenching refers to any process which decreases the fluorescent intensity of a given substance.
Quench is a verb that means to put out, cool, or satisfy something. It can also mean to terminate or relieve something. See synonyms, examples, and word history of quench.
Learn about the theory and applications of fluorescence quenching, a process that reduces the fluorescence intensity of a molecule by interaction with another species. Find chapters and articles on dynamic and static quenching, binding constants, fluorescent sensors and imaging agents, and more.
Fluorescence Quencher A fluorescence quencher, or ‘dark fluorophore,’ is a molecule that absorbs the photon emitted from a fluorophore but does not emit energy in the form of light, thereby suppressing fluorescence emission.
Quenching occurs via two distinct pathways. Collisional quenching occurs when the excited state fluorophore is deactivated upon contact with some quencher molecule in solution. Static quenching occurs when a fluorophore forms a non-fluorescent complex with a quencher and is no longer excitable.
Learn about the processes and applications of fluorescence quenching, which refers to any decrease in fluorescence intensity due to molecular interactions. This chapter covers collisional, static, and apparent quenching, and their implications for biochemical systems.
Most re-emit much of the energy as visible light. Others, called dark quenchers, do not have native fluorescence and instead emit the energy as heat. Examples of common quenchers include iodide ions, molecular oxygen and acrylamide..
