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Product Details. QuantiFast Multiplex PCR Kits enable fast and reliable quantification of up to 4 cDNA or gDNA targets in a single tube by multiplex, real-time PCR or two-step RT-PCR. Q-bond technology and an optimized master mix promote fast multiplex real-time PCR, not only on fast cyclers with short ramping times, but also on standard cyclers.
Taq DNA polymerase is the most commonly used enzyme in standard end-point PCR. It is isolated from the eubacterium Thermus aquaticus. This enzyme's robustness makes it suitable for a wide variety of PCR assays. However, since Taq DNA polymerase is active at room temperature, performing a reaction setup on ice is necessary to prevent nonspecific ...
The QIAcuity Residual DNA Quantification Kits provide accurate CHO, E.coli and HEK293 residual DNA (resDNA) quantification results with or without extraction, even in the presence of PCR contaminants or other inhibitory reagents. The assays are multicopy target assays that accurately determine highly fragmented residual host cell DNA.
QuantiTect Multiplex PCR Kits enable success in multiplex, two-step RT-PCR on the first attempt (see flowchart " QIAGEN multiplex kits "). The optimized master mix ensures that PCR products in a multiplex reaction are amplified with the same efficiency and sensitivity as PCR products in a corresponding single-amplification reactions.
QuantiNova Multiplex PCR Kits contain ready-to-use 4x master mixes that eliminate the need for optimization of reaction and cycling conditions. Simply add up to 800 ng template DNA and primer-probe sets to the master mix and follow the protocol in the handbook to get fast and reliable results on any real-time cycler.
Webinar recap: Introduction to digital PCR on the QIAcuity February 16, 2021. What are the fundamental aspects of performing a successful digital PCR (dPCR) assay on the QIAcuity instruments? That was the subject of a 2020 webinar featuring Dr. Andreas Missel, Director R&D at QIAGEN.
The MinElute PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products 70 bp – 4 kb in size. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An optional pH indicator allows easy ...
