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Primer3 is a widely used program for designing PCR primers (PCR = "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers. PCR is used for many different goals.
Primer3 picks primers for PCR reactions, considering as criteria: o oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities, o PCR product size, o positional constraints within the source (template) sequence, and o possibilities for ectopic priming (amplifying the wrong sequence) o many other constraints.
There are several available versions of the Primer3 web interface: Primer3Web and Primer3Plus. Primer3Web. Primer3Web is the original web-interface to Primer3. Primer3Web was developed by Steve Rozen and Helen Skaletsky. Primer3Web is maintained by Triinu Koressaar and Maido Remm. Primer3Web at primer3.ut.ee. Primer3Plus
Primer3 - Releases. The information below is meant to provide an "easy" means of locating and downloading the code/binaries you need to run Primer3. As such, we have provided links to help you reach the most recent versions of the software.
Problems using primer3, primer3web or primer3plus? We do not have the resources to provide end-user support. Nevertheless, you can send us your problem description and we will update the manual at this web site to address this problem.
Impressum. This homepage is maintained by the international Primer3 developper team. The core team is currently formed by Andreas Untergasser, Triinu Koressaar, Maido Remm and Steve Rozen. The pages are hosted at GitHub pages.
The Primer3 developper team takes privacy and the privacy of your data very seriously. We do not collect or store any personal information. This site is hosted on servers by Github Inc., 88 Colin P Kelly Jr. St, San Francisco, CA 94107, USA. We have no control over this server and how GitHub collects infromation.
