Search results
Feb 11, 2019 · Congo red agar (CRA) method that is a qualitative assay for detection of biofilm producer microorganism, as a result of color change of colonies inoculated on CRA medium, is described by Freeman et al.
Mar 1, 2013 · One method is based on culture in brain heart infusion agar (BHIA) containing sucrose and red Congo dye (original Congo red agar). Our group created a new CRA formula and we have confirmed its capacity to detect biofilm production in 210 S. epidermidis strains, including 76 (36.2%) icaAB gene–positive strains.
Aug 8, 2018 · To evaluate three in vitro phenotypic methods; tissue culture plate, tube method, and Congo red agar for detection of biofilm formation in staphylococci and assess the relation of biofilm formation with methicillin resistance and anti-microbial resistance.
Aug 29, 2020 · Congo Red Agar method. Freeman et al., 1989 described a simple qualitative method to detect the biofilm formation by Congo Red Agar (CRA) method. This method involves use of special media that is Brain Heart Infusion (BHI) agar with Sucrose and Congo red in the following composition: BHI agar-52 g/L; sucrose-36 g/L; agar-10 g/L; congo red-0.8 g ...
May 17, 2013 · The CRA plate test uses a solid medium, namely Congo red agar. This method allows for the direct analysis of the colonies and the identification of slime-forming strains (which appear as black colonies on the red agar) and non-slime-forming strains (red-coloured colonies).
Nov 1, 2016 · The Congo red agar method is widely used for identifying slime-producing or biofilm-forming Staphylococcus strains. However, these Congo red agar methods did not accurately identify slime-producing ica-positive strains regardless of their chemical compositions.
Jul 1, 2009 · Use of highly selective or differential medium, published Congo Red Agar (PCRA) has important shortcomings such as variations in black pigment formation while the intracellular adhesion locus...
