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Safranin ( Safranin O or basic red 2) is a biological stain used in histology and cytology. Safranin is used as a counterstain in some staining protocols, colouring cell nuclei red. This is the classic counterstain in both Gram stains and endospore staining.
The Gram stain uses the following dyes/reagents: crystal violet, Gram's iodine, ethanol, and safranin. The Gram stain distinguishes cells by cell wall type (Gram-positive or Gram negative). Gram-positive cells stain purple/violet.
The safranin is employed as a counter-stain in endospore staining and Gram’s staining. It is mostly utilized for the identification of cartilage, mucin, and mast cell granules. The safranin stain works by binding to acidic proteoglycans in cartilage tissues with a high affinity forming a reddish orange complex.
Application of counterstain (safranin): The red dye safranin stains the decolorized gram-negative cells red/pink; the gram-positive bacteria remain purple. Find information and process for the Preparation of Gram Staining Regent
3.2 A standard safranin experiment. Safranin is a lipophilic cationic dye which accumulates in mitochondria according to the inside negative potential in energized mitochondria.
May 15, 2022 · Safranin (SAF, also known as safranin O, safranin T, and Basic red 2) and phenosafranin (PheSAF, Fig. 1) are classic phenazinium dyes. Despite the structural similarity, their applications, in addition to the initiation of photopolymerization [1] and redox indicators [2], are still different.
Jul 31, 2022 · Safranin (also referred to as safranin O or basic red 2), a less expensive and safer plant histology stain, could potentially be utilized for human tissues since it provides equivalent or better accuracy in the diagnosis of frozen sections of basal and squamous cell carcinomas. 10
The analysis of OXPHOS in mouse brain homogenates revealed that, at commonly applied concentrations, safranin inhibits Complex I-driven OXPHOS capacity, primarily targeting the phosphorylation system, but has no effects on LEAK respiration.
Dyes commonly used for staining cartilage glycosaminoglycans take advantage of the negative charge of molecules, and these include Safranin O, toluidine blue, and Alcian blue (Figure 5.17). Safranin O is a red to orange stain, often used with Fast Green (a neutral dye) as a counterstain.
Oxygen consumption is simultaneously measured with safranin O reflecting Δ Ψm or MgGr fluorescence reflecting ADP–ATP exchange rates in the same samples polarographically using the Oxygraph-2k.
