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  1. vlab.amrita.eduDesigning a primer - Amrita Vishwa Vidyapeetham Virtual Lab

    3 days ago · Searches for possible primers that matches with the length, GC content, Tm etc. which user specifies. Reads file formats such as FASTA, Genbank, EMBL and formats from DNA Strider. Save files in Genbank format or DNA Strider-compatible format. Allows direct BLAST search in NCBI. Highlights restriction sites from the input.

    • Procedure

      Copy a nucleotide FASTA sequence from NCBI. This experiment...

  2. aacrjournals.org › cancerres › articleTargeting Cholesterol Biosynthesis with Statins Synergizes with...

    1 day ago · For qRT-PCR primers, which were designed using the NCBI Primer-BLAST tool, see Supplementary Table S2. Primer efficiency was confirmed to be within 90% to 110% for each primer pair in each cell line.

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  4. link.springer.com › article › 10XylR regulates genes at xyl cluster, involved in D-xylose ... -...

    1 day ago · The primers used in this work (Table S1) were designed using NCBI Primer-BLAST program (Ye et al. 2012)d seropedicae Z69 genome (GenBank: CP049334) as template (Malán et al. 2021). Sequence analysis was done using Blastp ( https://blast.ncbi.nlm.nih.gov/Blast.cgi ).

  5. vlab.amrita.eduDesigning a primer (Procedure) : Bioinformatics Virtual Lab I ...

    4 days ago · Copy a nucleotide FASTA sequence from NCBI. This experiment shows an example with insulin FASTA sequence of Drosophila Melanogaster. Figure 1: Paste the FASTA sequence . Now select the sequence. Figure 2: Selecting the sequence . To search for primers, go to "Tools --> Find Primers". Figure 3: Searching for primers

  6. www.sciencedirect.com › science › articleEvaluation of in vitro probiotic properties and colonization of...

    5 days ago · Subsequently, unassigned genes of B. laterosporus S62-9 were analyzed by the BLASTN search at the NCBI to screen for unique genes. All primer pairs were designed using Primer-Blast (https://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?LINK_LOC=BlastHome).

  7. bmcplantbiol.biomedcentral.com › articles › 10Isolation and functional analysis of the Larix olgensis LoNAC3...

    4 days ago · Primers were designed based on the NCBI Larix kaempferi genome (taxid: 54800, L. kaempferi GenBank assembly GCA_013171265.2 Nucleotide BLAST): Forword primers (5’-3’) GCCCCGTTTGTCGTAG; Reverse primers (5’-3’) CATGTCCTGTAAACAGAAAACAGGATTT.

  8. www.cell.com › cell › fulltextDetection and analysis of complex structural variation in human...

    2 days ago · For each cxSV, a primer pair was designed to amplify all breakpoints in one PCR reaction. For heterozygous calls, we ensured that the reference allele was also amplified to confirm genotype. Sequencing from both forward and reverse primers yielded validation rates of 90% (9/10) for HuRef and 85% (11/13) for HepG2 .