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Nick translation (or head translation), developed in 1977 by Peter Rigby and Paul Berg, is a tagging technique in molecular biology in which DNA Polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues, creating a tagged DNA sequence which can be used as a probe in fluorescent in situ hybridization ...
22 Jul 2020 · Nick translation is a tagging technique where DNA polymerase I is used to replace some of the nucleotides of a DNA sequence with their labeled analogues. In this process, DNA molecules are first treated with DNase to produce single-stranded “nicks”.
The nick translation reaction is used to introduce radioactive nucleotide phosphates into unlabeled DNA for the purpose of making a probe. The reaction depends on the ability of the enzyme DNA polymerase I to initiate DNA synthesis at free 3´ OH groups, which are exposed as nicks in the unlabeled DNA.
Nick translation is a biological process in which a single-stranded DNA nick serves as the marker for DNA polymerase to excise and replace possibly damaged nucleotides. At the end of the segment that DNA polymerase acts on, DNA ligase must repair the final segment of DNA backbone in order to complete the repair process. [4]
Nick translation is a method of labeling DNA with radioactive or biotin moieties for hybridization analysis. It involves nicking the DNA with DNase I and replacing the native nucleotides with labeled precursors by DNA polymerase I.
Nick translation is one method of labeling DNA to be used as a hybridization probe. This method uses the enzymes pancreatic DNase I and Escherichia coli DNA polymerase I. Under the nick translation reaction conditions that use Mg 2+, DNase I randomly nicks double-stranded DNA to leave 5′ phosphate termini. A nick is a break in a ...
Learn how to label DNA with radioactive nucleotides using nick translation, a method that nicks and extends the DNA strand with DNA polymerase I. Follow the step-by-step instructions, materials, and tips for optimal probe quality and safety.
