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May 18, 2022 · Learn how to perform Kirby Bauer disc diffusion test for antibiotic susceptibility testing of aerobes or facultatively anaerobes. Find out the aim, principle, materials, procedure, result interpretation, uses, advantages and limitations of this technique.
The purpose of the Kirby-Bauer disk diffusion susceptibility test is to determine the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds in order to assist a physician in selecting treatment options for his or her patients.
In Kirby-Bauer testing, bacteria are placed on a plate of solid growth medium and wafers of antibiotics (white disks, shown) are added to the plate. After allowing the bacteria to grow overnight, areas of clear media surrounding the disks indicate that the antibiotic inhibits bacterial growth.
The disk diffusion test (also known as the agar diffusion test, Kirby–Bauer test, disc-diffusion antibiotic susceptibility test, disc-diffusion antibiotic sensitivity test and KB test) is a culture -based microbiology assay used in diagnostic and drug discovery laboratories.
Kirby-Bauer Test. The results of the Kirby-Bauer Test provide an accurate prediction of which antibiotics are likely to be effective against the pathogen. Because the Kirby-Bauer test is relatively simple to perform and is inexpensive, it has been extensively used in medical practice.
Dec 24, 2022 · Kirby-Bauer antibiotic testing (also called KB testing or disk diffusion antibiotic sensitivity testing) uses antibiotic-containing wafers or disks to test whether particular bacteria are susceptible to specific antibiotics. First, a pure culture of bacteria is isolated from the patient.
Dec 8, 2009 · The Kirby-Bauer disk diffusion susceptibility test determines the sensitivity or resistance of pathogenic bacteria to various antimicrobial compounds in order to assist physicians in selecting treatment options their patients.
