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Mar 10, 2018 · One of the classic ways to determine the concentration of microbes in a sample is to dilute the sample, grow the microbes on plates and count the colonies. The plated microbes grow from a colony forming unit consisting of one or more cells into a visible colony that can be seen and counted.
You will avoid counting a colony twice by marking off the colonies on the bottom of the plate as you count them. This requires, of course, that the plate be upside down. Be sure to count any small colonies.
The number of colonies we count on a petri plate enables us to calculate the CFU or colony forming units. CFU is a measure of the concentration of the live, viable bacterial cells capable of reproducing when grown on a petri plate in cells per milliliter (cells/mL).
The most common method of measuring viable bacterial cell numbers is the standard or viable plate count or colony count. This is a viable count, NOT a total cell count. It reveals information related only to viable or live bacteria.
Jan 3, 2022 · Count colonies on all plates containing 15-300 colonies. Report the count as CFU/ml for the liquid sample and CFU/g for the solid sample allowing for dilution factors. Bacillus subtilis = Growth, straw-colored colonies.
Dec 12, 2016 · Instructors explain the concept of serial dilution, as well as the process of finding that dilution which provides a readily countable number of colonies on a plate. Most textbooks and lab manuals typically recommend using plates that yield somewhere between 30 and 300 colonies (or some similar range).
How to count your plates. 1. Upload an image of your agar plate. 2. Draw a circle to outline the plate and trim the edges. 3. Select the colony type you wish to count. 4. Click “Count Colonies” 5. Adjust the threshold and minimum/maximum colony size. 6. Download your results!
