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Feb 12, 2023 · Typical examples are the total length of the cell cycle, the length and variability of distinct cell cycle phases, the presence of quiescent or senescent cells, or the presence of death cells. This review is focused exclusively on the basic techniques of cell cycle analysis in asynchronous cell population.
- Assaying cell cycle status using flow cytometry - PMC
Assessing cell cycle distribution and cell proliferation is...
- The palette of techniques for cell cycle analysis - PMC
Measuring the cell cycle can include probing many aspects:...
- Assaying cell cycle status using flow cytometry - PMC
Learn how to measure cell cycle stages using flow cytometry or imaging with dyes, kits, and antibodies. Also, detect senescent cells with a fluorescent probe that activates β-galactosidase.
Assessing cell cycle distribution and cell proliferation is important for studying cell growth differentiation, senescence and apoptosis. This enables one to investigate underlying basic mechanisms as well as to evaluate therapeutic efficacies of anti-cancer drugs.
Cell cycle analysis by DNA content measurement is a method that most frequently employs flow cytometry to distinguish cells in different phases of the cell cycle.
Learn how to use flow cytometry to estimate the percentages of cells in different phases of the cell cycle, or to analyze just the S phase with EdU labeling. Compare different DNA stains for live-cell cycle analysis and see their advantages and limitations.
Measuring the cell cycle can include probing many aspects: whether or not a cell is cycling or whether it retains the potential to cycle; which cell cycle phase a cell is in, or whether it has progressed beyond a given point of interest, such as a checkpoint or the completion of DNA replication; the dwell-time in a particular phase; the number o...
Sep 1, 2022 · With the recent advances in DNA dyes, understanding of additional cell cycle phase markers, and new technologies, cell cycle analysis continues to be a dynamic field within the flow cytometry community. This chapter will give an overview of the current state of cell cycle analysis by flow cytometry.
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