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Durham tubes are used in microbiology to detect production of gas by microorganisms. They are simply smaller test tubes inserted upside down in another test tube so they are freely movable.
This tiny upside down tube is called a Durham tube. If gas is produced during fermentation, there will be a space in the top of the Durham tube that does not have any medium in it since gas has displaced the medium at the top of the tube forming a bubble.
Small inverted tubes called Durham tube is also immersed in the medium to test for the production of the gas (hydrogen or carbon dioxide). The term fermentation is often used to describe the breaking down or catabolism of a carbohydrate under anaerobic conditions.
To detect these gases, a Durham tube is used. This is a small inverted glass tube that is placed within the larger glass tube containing the fermentation medium (see image 1). If gases (typically CO 2) are produced during the fermentation process, a bubble will form at the top of the Durham tube (see tube A). If you see a bubble in the Durham ...
The Durham tube method is used to detect production of gas by microorganisms. They are simply smaller test tubes inserted upside down in another test tube. This small tube is initially filled with the solution in which the microorganism is to be grown.
Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria. It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose).
Dispense the double strength medium in 5 tubes (10mL in each tube) and 50 mL single strength medium in 1 bottle and add a Durham tube in an inverted position. Examine the tubes to make sure that the inner vial is full of liquid with no air bubbles.
