Search results
Primer3 was a complete re-implementation of an earlier program: Primer 0.5 (Steve Lincoln, Mark Daly, and Eric S. Lander). Primer3 web service at primer3.ut.ee is a part of services provided by ELIXIR - European research infrastructure for biological information.
Primer3 is a program for designing PCR primers (PCR = "Polymerase Chain Reaction"). PCR is an essential and ubiquitous tool in genetics and molecular biology. Primer3 can also design hybridization probes and sequencing primers. PCR is used for many different goals.
Primer3Plus picks primers from a DNA sequence using Primer3. This is the latest version straight from the developers with all the new features.
There is a newer version of Primer3 available at http://primer3.ut.ee. Paste source sequence below (5'->3', string of ACGTNacgtn -- other letters treated as N -- numbers and blanks ignored). FASTA format ok.
Pick hybridization probe. (internal oligo) or use oligo below. Pick right primer or use right primer. below (5'->3' on opposite strand).
Primer3Plus is an updated, task-oriented web-interface to Primer3. Primer3Plus is developed and maintained by Andreas Untergasser. Primer3Plus at www.primer3plus.com
Primer3 picks primers for PCR reactions, considering as criteria: o oligonucleotide melting temperature, size, GC content, and primer-dimer possibilities, o PCR product size, o positional constraints within the source (template) sequence, and o possibilities for ectopic priming (amplifying the wrong sequence) o many other constraints.
Jan 26, 2022 · Download Primer3 - PCR primer design tool for free. Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3").
Primer3 converts concentration of divalent cations to concentration of monovalent cations using formula suggested in the paper Ahsen et al., 2001. [Monovalent cations] = [Monovalent cations] + 120*(v([divalent cations] - [dNTP])).
Design PCR primers from DNA sequence. Widely used (190k Google hits for "primer3"). From mispriming libraries to sequence quality data to the generation of internal oligos, primer3 does it. C&perl.
