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Select “Analysis” and then method “CF - Crude fiber (Weende method)” including the following parameters: • Crucibles porosity P2 • Preheat: Yes • Enzyme: No • H 2 SO 4: 150 ml • N-octanol: Yes • Digestion time: 30 minutes • Washing: 3 x 50 ml of distilled water • KOH: 150 ml • Digestion time: 30 minutes
The composition of nutritional values within the animal feed sector is often determined with the Weende analysis. In this method different substances are summarized in groups.
In the so-called Weende method, (fig. 1) Crude Pro-tein, Crude Fat and Crude Ash are determined and the moisture and Carbohydrate content can then be calculated by difference: Carbohydrates = amount of total sample – Moisture – Crude Protein – Crude Fat.
It is often referred to as the Weende System and was principally devised to separate carbohydrates into two fractions: crude fibre and nitrogen free extract (NFE). The system consists of determinations of water, ash, crude fat (ether extract), crude protein and crude fibre.
This procedure is used for the determination of crude fiber (CF) in animal feed and pet food using the Weende method (AOAC method 978.10). Using sequential acid and alkali extractions, this method predominantly removes protein, sugar, starch, lipids, and portions of both structural carbohydrates and lignin.
Fiber Determination in Oatmeal according to Weende method The method is based on the solubilization (digestion) of non-cellulosic compounds by sulfuric acid and potassium hydroxide solutions. Crude fiber is the loss on ignition of the dried residue remaining after digestion of the sample and determined by weight difference.
The method is based on drying a sample in an oven and determining moisture content by the weight difference between dry and wet material. Apparatus. Drying oven. Dryers. Method. Weigh out approx. 5–10 g of previously ground sample. Place sample in drying oven at 105°C for at least 12 h. Let sample cool in dryer.
