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  1. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches.

    • Nucleotide Blast

      PSI-BLAST allows the user to build a PSSM (position-specific...

    • Home

      The Basic Local Alignment Search Tool (BLAST) finds regions...

    • Recent Results

      This page provides links to your unexpired BLAST jobs. Enter...

    • Saved Strategies

      clicking the 'Saved Search Strategy' link at the top of a...

    • Help

      Welcome to BLAST Help¶ Getting started¶ Web BLAST quick...

    • Tblastn

      Enter one or more queries in the top text box and one or...

    • Smartblast

      Smart BLAST is a tool for searching and aligning protein...

    • BLASTHelp Documentation

      The NCBI supports RESTful interface to programmatically...

  2. Select the sequence database to run searches against. No BLAST database contains all the sequences at NCBI. BLAST databases are organized by informational content (nr, RefSeq, etc.) or by sequencing technique (WGS, EST, etc.). more...

  3. The program compares nucleotide or protein sequences to sequence in a database and calculates the statistical significance of the matches. This chapter first provides an introduction to BLAST and then describes the practical application of different BLAST programs based on the BLAST Quick Start mini-course (www.ncbi.nlm.nih.gov/Class/minicourses).

  4. Learn about the new features and enhancements of BLAST, the popular tool for sequence similarity search. Find links to BLAST forms, databases, documentation, and recent results.

  5. Jun 23, 2008 · This manual documents the BLAST (Basic Local Alignment Search Tool) command line applications developed at the National Center for Biotechnology Information (NCBI).

  6. BLAST is available on the web on the NCBI website. Different types of BLASTs are available according to the query sequences and the target databases. Alternative implementations include AB-BLAST (formerly known as WU-BLAST), FSA-BLAST (last updated in 2006), and ScalaBLAST.

  7. Aug 6, 2024 · BLASTp (Protein BLAST): compares one or more protein query sequences to a subject protein sequence or a database of protein sequences. This is useful when trying to identify a protein (see From sequence to protein and gene below).