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- Dictionarycomplete/kəmˈpliːt/
adjective
- 1. having all the necessary or appropriate parts: "a complete list of courses offered by the university"
- 2. (often used for emphasis) to the greatest extent or degree; total: "a complete ban on smoking" Similar Opposite
verb
- 1. finish making or doing: "he completed his PhD in 1993" Similar Opposite
- 2. provide with the item or items necessary to make (something) full or entire: "complete your collection of Britain's brightest gardening magazine" Similar
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cds means coding sequence. as you can see in your file under features - source - it says "genomic DNA". and it contains the intron 347 - 660 (see mRNA in the file and CDS) as well as 5' and 3' UTR which cDNA wouldn't. -kylvalda-.
Jul 9, 2008 · If after 18-24hr, nocodazole causes complete 4N arrest, but your drug doesn't, you can at least say your drug is less efficient at G2/M arrest (or perhaps your drug is affecting the progression at another point in the cycle as well). However, if after Nocodazole treatment you still see 20% of the cells sitting at G1, you will know that you can probably only hope to get 80% arrest with your ...
Oct 18, 2006 · Protocol: Remove and discard culture medium. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes ...
I think gelatin is somehow crude and not well defined for the composition of collagen types or any other components; so, there might be differences in gelatin quality between providers and even charges; if there is no need to exactly define the coat, to say "collagen I" or "collagen IV", why not a trial with gelatin...however, I would look for effects of different charges
Oche. -oche-. 1. A strain is a bacterium that has some level of genetic definition, often plasmids that confer specific traits. An isolate, by contrast does not necessarily have such definition, but is simply the bugs that were isolated from the sample. 2.
So I guess they should be stable for 3-4 days. -scolix-. Well I can only say that I'm using my medium, which also contains human bFGF, for about a week. -Tabaluga-. I've kept various growth factors for up to a week in media previously - but the only reason for stopping there was the duration of the experiment. It wasn't a concern I'd considered ...
This version of the Sequence Manipulation Suite represents a complete re-write of the previous version. The new version is much faster and has many new features. The previous version of the Sequence Manipulation Suite can still be accessed. Send questions and comments to stothard@ualberta.ca. new window | home | citation 2.4.4-Sat Jan 31 06:07: ...
There is another calculation which help define if a stretch of DNA is a CpG island or not. It is called the ratio of observed CpGs over expected CpGs. Check this thread if you want to know more It is called the ratio of observed CpGs over expected CpGs.
Jul 1, 2008 · I always add AEBSF (1mM), Complete (Roche) 1X, sodium vanadate, a tyrosine phophatase inhibitor, and sodium fluoride, a ser/thr phosphatase inhibitor. Although I've seen quite a wide range of concentrations in different protocols/recipes, personally I use them at 2mM and 10mM respectively. I've seen concentrations as high as 200mM and 50mM though.
Molecular biology protocol: DNA:transformation . Protocols. Transformation (Gottschling Lab, Fred Hutchinson Cancer Research Center)
